Frequent Questions

What column should be used as the confirmation column for SW-846 Method 8310?

Method 8310, the high performance liquid chromatographic (HPLC) method for Polynuclear Aromatic Hydrocarbons (PAHs), does not list a confirmation column. What column should I use to confirm my results from the primary column?

 

The following columns have been demonstrated to be appropriate for the analysis of PAHs by HPLC:  

• Standard analytical column - C-18 reversed-phase column, 3-5 µm particle size, 100-250 mm x 4.6 mm ID, (Perkin-Elmer ChromSPHERE-3-PAH, Supelco Supelcosil LC-PAH, or equivalent). 

• Narrow-bore analytical column - C-18 reversed-phase column, 3-5 µm particle size, 100-250 mm x 2.1 mm ID, (Supelco Supelcosil LC-PAH, or equivalent). 

• Confirmation column - CN reversed-phase column, 3-5 µm particle size, 150-250 mm x 3.0-4.6 mm ID, (Supelco Supelcosil LC-PCN, Waters Nova-Pak CN HP, or equivalent). 

Other HPLC columns also may be available to provide a sufficient change in elution order to be useful for confirmation. 

Finally, you should consult Method 8000D for information on confirmation analyses, including when confirmations are needed and when they may not be needed.

 

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